Three RFLPs for the insulin receptor gene INSR: EcoRI, Pst I, Hind III.
نویسندگان
چکیده
SOURCE AND DESCRIPTION OF CLONE: A 973 bp cDNA insert from an adult human kidney library cloned in the EcoRI site of PBR 327 (pINSR 18.2) encoding aminoacids-26 to 295 of the insulin receptor. POLYMORPHISM EcoRI identifies a two allele polymorphism with bands at 22 fcb and 20 Kb Hind III detects two allelic fragments of 6.5 Kb and 1.4 kb Pst I reveals two allelic fragments of 2.
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SOURCE/DESCRIPTION A 4.2 kb EcoRI fragment cloned i n pBR 327 (pINSR 1 3-1 , Shaw e t a l. , 1985) , which covers amino a c i d 295 t o t h e poly A t r a c t of t h e human i n s u l i n r e c e p t o r gene (U l l r i c h e t a l. , 1985). polymorphism with a band a t 4.4 kb or 3.8 k b , and s e v e r a l c o n s t a n t bands. MENDELIAN INHERITANCE Co-dominant segregation observed in 1 infor...
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 14 16 شماره
صفحات -
تاریخ انتشار 1986